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刊物信息
期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
编辑部主任:粟晓黎
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237

 

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HPLC法测定复方黄芩片中6个成分的含量

Simultaneous determination of six components in Fufang Huangqin tablets by HPLC

作者: 唐德智 
作者(英文):TANG De-zhi
分类号:
出版年·卷·期(页码):2016,36 (10):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立HPLC波长切换法测定复方黄芩片中虎杖苷、黄芩苷、汉黄芩苷、黄芩素、穿心莲内酯、脱水穿心莲内酯的含量。方法:采用Agilent SB-C18(4.6 mm×150 mm,5 μm)色谱柱,流动相为乙腈(A)-0.1磷酸水溶液(B)系统,梯度洗脱,流速为1.0 mL·min-1,柱温:30℃,波长切换时间序列采样(0~10 min,306 nm,检测虎杖苷;10~23 min,280 nm,检测黄芩苷;23~25min,225 nm,检测穿心莲内酯;25~33 min,280 nm,检测汉黄芩苷、黄芩素;33~50 min,254 nm,检测脱水穿心莲内酯)。结果:虎杖苷进样量在0.046~0.823 μg范围内线性关系良好(r=0.999 9,n=6),平均回收率为99.30%,RSD为1.5%(n=6);黄芩苷进样量在0.468~8.428 μg范围内线性关系良好(r=0.999 7,n=6),平均回收率为99.76%,RSD为1.3%(n=6);汉黄芩苷进样量在0.116~2.084 μg范围内线性关系良好(r=0.999 9,n=6),平均回收率为100.3%,RSD为1.8%(n=6);黄芩素进样量在0.117~2.110 μg范围内线性关系良好(r=0.999 8,n=6),平均回收率为99.25%,RSD为1.3%(n=6);穿心莲内酯进样量在0.029~0.524 μg范围内线性关系良好(r=0.999 8,n=6),平均回收率为99.91%,RSD为1.6%(n=6);脱水穿心莲内酯进样量在0.033~0.592 μg范围内线性关系良好(r=0.999 7,n=6),平均回收率为99.94%,RSD为1.6%(n=6)。5批样品中虎杖苷、黄芩苷、汉黄芩苷、黄芩素、穿心莲内酯和脱水穿心莲内酯含量范围分别为2.09~2.25、22.74~25.91、5.69~6.08、5.74~6.11、1.27~1.41、1.49~1.69 mg·g-1结论:该方法经方法学验证,可用于复方黄芩片的质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for simultaneous determination of polydatin, baicalin, wogonoside, baicalein, andrographolide, and dehydroandrographolide in Fufang Huangqin tablets. Methods: The separation was performed on an Agilent SB-C18 column(4.6 mm×150 mm, 5 μm) with gradient elution at a temperature of 30℃. The acetonitrile and 0.1%(v/v) phosphoric acid were used as the mobile phase with a flow rate of 1.0 mL·min-1. The detection wavelength was 306 nm(0-10 min) for polydatin,280 nm(10-23 min) for baicalin, 225 nm(23-25 min) for andrographolide, 280 nm(25-33 min) for wogonoside and baicalein,and 254 nm(33-50 min) for dehydroandrographolide. Results: Polydatin had a good linearity in the range of 0.046-0.823 μg,the average recovery was 99.30% with RSD of 1.5%(n=6);baicalin had a good linearity in the range of 0.468-8.428 μg, the average recovery was 99.76% with RSD of 1.3%(n=6);wogonoside had a good linearity in the range of 0.116-2.084 μg,the average recovery was 100.3% with RSD of 1.8%(n=6);baicalein had a good linearity in the range of 0.117-2.110 μg,the average recovery was 99.25% with RSD of 1.3%(n=6);andrographolide had a good linearity in the range of 0.029-0.524 μg,the average recovery was 99.91% with RSD of 1.6%(n=6);dehydroandrographolide had a good linearity in the range of 0.033-0.592 μg,the average recovery was 99.94% with RSD of 1.6%(n=6).The contents of polydatin,baicalin,wogonoside,baicalein,andrographolide and dehydroandrographolide in 5 samples were 2.09-2.25, 22.74-25.91,5.69-6.08,5.74-6.11,1.27-1.41 and 1.49-1.69 mg·g-1,respectively. Conclusion: The established method is validated by methodology that it is suitable for the quality control of Fufang Huangqin tablets.

-----参考文献:---------------------------------------------------------------------------------------
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