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刊物信息
期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
编辑部主任:粟晓黎
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237

 

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高效液相色谱法测定蒲公英提取物中绿原酸含量

HPLC determination of chlorogenic acid in dandelion extract

分类号:
出版年·卷·期(页码):2009,29 (7):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立HPLC法测定蒲公英提取物中绿原酸含量。方法:采用Agilent Zorbax SB C18柱(4.6mm×250mm,5μm),流动相为乙腈0.4%磷酸溶液(1090),检测波长324 nm,柱温30,流速l.0 mL·min-1结果:绿原酸的线性范围为0.040 -----英文摘要:---------------------------------------------------------------------------------------

 Abstract  Objective:To establish an HPLC method for the determination of chlorogenic acid in dandelion extract. Method:Use Agilent Zorbax SB C18 column4.6mm×250mm,5μmwith the mobile phase consisting of acetonitrile0.4phosphoric acid solution 1090,detected wavelength was set at 324 nm,column temperature 30flow rate at l.0 mL·min-1Result:The linear ranges of chlorogenic acid  was 0.0400.60 μgr=0.9992.The average recoveries was 96.48%, RSD was1.90Conclusion: HPLC determination of chlorogenic acid in dandelion extract is rapid,simple and accurate. It can be applied to determine of chlorogenic acid in dandelion extract.

-----参考文献:---------------------------------------------------------------------------------------

 

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4.       LIU Jing(刘净),LIANG Jing-yu(梁敬钰), XIE Tao(谢韬). Development of iridoids in rencent years(环烯醚萜类化合物近年研究进展). Strait Pharm J (海峡药学),2004,16(1):14-19.

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10.   LING Yun(凌云), BAO Yan-yan(鲍燕燕),ZHANG Yong-lin(张永林), etal. Study on the chemical constituents of Xing An Taraxacum (兴安蒲公英的化学成分研究).J Naval Gen Hosp(海军总医院学报),1999,12(4):233-235.

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