关闭
 
读者在线:用户名 密码
首页 期刊简介 投稿须知 期刊目录 专家风采 编委会 特邀顾问 联系我们 移动出版
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5



刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

访问统计
您是第  4 2 9 8 4 7 6 位浏览者
您当前的位置:首页 >> 正文

UPLC-MS/MS法同时测定金花葵中11个活性成分

Simultaneous determination of 11 active components in Aurea helianthus by UPLC-MS/MS

作者(英文):
分类号:R917
出版年·卷·期(页码):2019,39 (5):780-786
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立UPLC-MS/MS法同时测定金花葵中11个活性成分(没食子酸、原儿茶酸、绿原酸、咖啡酸、芦丁、金丝桃苷、异槲皮苷、棉皮素、槲皮素-3'-O-葡萄糖苷、杨梅素、槲皮素)的含量。方法:色谱条件:采用Waters ACQUITY BEN C18(2.1 mm×100 mm,1.7 μm)色谱柱,以0.05%甲酸水-乙腈为流动相,梯度洗脱,流速为0.2 mL·min-1,柱温为30℃。质谱条件:采用电喷雾电离源(ESI),正负离子全扫模式(Full MS/dd-MS2)扫描进行定量分析。结果:金花葵中没食子酸、原儿茶酸、绿原酸、咖啡酸、芦丁、金丝桃苷、异槲皮苷、棉皮素、槲皮素-3'-O-葡萄糖苷、杨梅素、槲皮素的质量浓度分别在0.030 64~0.153 2、0.044 00~0.220 0、0.144 0~0.720 0、0.026 64~0.133 2、6.352~31.76、8.704~43.52、5.728~28.64、24.30~121.5、5.824~29.12、2.248~11.24、0.7264~3.632 μg·mL-1范围内线性关系良好(R2 ≥ 0.999 1),精密度、重复性、稳定性均良好,平均加样回收率在94.5%~100.7%之间,RSD ≤ 3.2%;8批金花葵样品中上述11个活性成分的含量范围分别为0.005~0.049、0.008~0.122、0.032~0.267、0.012~0.033、2.439~4.214、4.904~7.216、3.915~5.945、12.126~17.518、4.006~5.423、0.573~1.753、0.186~1.243 mg·g-1结论:该方法可用于金花葵药材的质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective:To establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 11 active components (gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, rutin, hyperin, isoquercitrin, hibifolin, quercetin-3'-O-glucoside, myricetin and quercetin) in Aurea helianthus.Methods:Chromatographic conditions:The chromatographic column was Waters ACQUITY BEN C18 (2.1 mm×100 mm, 1.7 μm).The mobile phase was composed of 0.05% formic acid water (A) and acetonitrile (B) in gradient elution at a flow rate of 0.20 mL·min-1 and the column temperature was set at 30℃.Mass spectrum conditions:The electrospray ionization source (ESI) and positive and negative ion monitoring mode (Full MS/dd-MS2) were used for quantitative analysis.Results:Good linearity (R2 ≥ 0.999 1) was achieved for gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, rutin, hyperin, isoquercitrin, hibifolin, quercetin-3'-O-glucoside, myricetin and quercetin in the range of 0.030 64-0.153 2, 0.044 00-0.220 0, 0.144 0-0.720 0, 0.026 64-0.133 2, 6.352-31.76, 8.704-43.52, 5.728-28.64, 24.30-121.5, 5.824-29.12, 2.248-11.24 and 0.726 4-3.632 μg·mL-1, respectively.The precision, repeatability, and stability were good in this method.The average recoveries varied between 94.5% and 100.7% with RSDs ≤ 3.2%.And the average contents of 11 active components in 8 batches of Aurea helianthus were 0.005-0.049, 0.008-0.122, 0.032-0.267, 0.012-0.033, 2.439-4.214, 4.904-7.216, 3.915-5.945, 12.126-17.518, 4.006-5.423, 0.573-1.753 and 0.186-1.243 mg·g-1.Conclusion:This method can be used for the quality control of Aurea helianthus.

-----参考文献:---------------------------------------------------------------------------------------

[1] 吴正平.金花葵花总黄酮对衰老模型小鼠抗氧化和免疫功能的影响[J].中国老年学杂志,2011,31(10):1800 WU ZP.Effect of total flavonoids of Hibiscus manihot L.flower on antioxidant and immune function of aging mice[J].Chin J Gerontol,2011,31(10):1800
[2] 张建.金花葵花总黄酮解热抗炎作用的实验研究[J].中国医科大学学报,2011,40(8):763 ZHANG J.Experimental study of antipyretic and anti-inflammatory effects of total flavonoids in Abelmoschus manihot[J].J China Med Univ,2011,40(8):763
[3] 王晓玉,魏文,何计国.金花葵黄酮对糖尿病模型小鼠脂代谢的影响[J].中国农学通报,2011,27(29):102 WANG XY,WEI W,HE JG.Effect of Abelmoschus manihot flavone on lipid metabolism in diabetic mice[J].Chin Agric Sci Bull,2011,27(29):102
[4] 李芸,杨秀松,何计国.金花葵黄酮对高脂血症大鼠血脂的影响[J].食品科学,2012,33(1):248 LI Y,YANG XS,HE JG.Effect of Abelmoschus manihot flavonoids on blood lipid levels of hyperlipidemic rats[J].Food Sci,2012,33(1):248
[5] 吴正平.金花葵籽不饱和脂肪酸对实验性高脂血症大鼠血脂和肝功能的影响[J].中成药,2011,33(7):1245 WU ZP.Effects of Abelmoschus manihot unsaturated fatty acids on blood lipid and liver function in experimental hyperlipidemia rats[J].Chin Tradit Pat Med,2011,33(7):1245
[6] 郑雪花,杨君,杨跃辉.没食子酸药理作用的研究进展[J].中国医院药学杂志,2017,37(1):94 ZHEGN XH,YANG J,YANG YH.Research progress on pharmacological effects of gallic acid[J].Chin J Hosp Pharm,2017,37(1):94
[7] 陈家全,王慧,李冬玉,等.原儿茶酸在大鼠体内代谢产物的分析[J].中成药,2017,39(3):561 CHEN JQ,WANG H,LI DY,et al.Analysis of metabolites of protocatechuic acid in rats in vivo[J].Chin Tradit Pat Med,2017,39(3):561
[8] 那袭雪,张文涛,谈远锋,等.绿原酸及其异构体药理作用及不良反应研究进展[J].辽宁中医药大学学报,2018,20(3):140 NA XX,ZHANG WT,TAN YF,et al.Research progress on the pharmacological effects and adverse reactions of chlorogenic acid and its isomers[J].J Liaoning Univ TCM,2018,20(3):140
[9] 孙皓熠,郝宝燕,张浩超,等.咖啡酸研究概况[J].食品与药品,2017,19(2):151 SUN HY,HAO BY,ZHANG HC,et al.Research situation of caffeic acid[J].Food Drug,2017,19(2):151
[10] 支旭然,刘洪涛,吴茵,等.UPLC-MS/MS法同时测定肾康注射液中7个有效成分[J].药物分析杂志,2017,37(1):37 ZHI XR,LIU HT,WU Y,et al.Simultaneous determination of 7 active constituents in Shenkang injection by UPLC-MS/MS[J].Chin J Pharm Anal,2017,37(1):37
[11] 黄秋妹,许舒瑜,邰艳妮,等.UPLC-MS/MS同时测定金牡感冒片中15个成分[J].药物分析杂志,2017,37(8):1453 HUANG QM,XU SY,TAI YN,et al.Simultaneous determination of fifteen components in Jinmu Ganmao tablets by UPLC-MS/MS[J].Chin J Pharm Anal,2017,37(8):1453
[12] 程巧鸳,王笑笑.UPLC-MS/MS法分析3种蜂蜜及其蜜源花中的特征黄酮类组分[J].药物分析杂志,2017,37(6):994 CHENG QY,WANG XX.Analysis of characteristic flavonoids in three kinds of honey and nectar flowers by UPLC-MS/MS[J].Chin J Pharm Anal,2017,37(6):994
[13] 赵振霞,王敏,王钰宁,等.UPLC-MS/MS法测定心可舒胶囊中5种皂苷类成分的含量[J].药物分析杂志,2016,36(3):494 ZHAO ZX,WANG M,WANG YN,et al.Simultaneous determination of five saponins in Xinkeshu capsules by UPLC-MS/MS[J].Chin J Pharm Anal,2016,36(3):494
[14] 冯朵,谭立,彭平,等.基于药物体系的金花葵特征图谱质量表征关联分析研究[J].北京中医药大学学报,2016,39(4):308 FENG D,TAN L,PENG P,et al.Quality representation and correlation analysis of Aurea helianthus based on characteristic chromatogram of drug system[J].J Beijing Univ Tradit Chin Med,2016,39(4):308
[15] 刘琦.金花葵花总黄酮提取、纯化、分离制备及抗氧化性研究[D].兰州:甘肃农业大学,2012 LIU Q.Study on the Extraction,Purification,Preparative Isolation and Antioxidant Activity of Totle Flavones of Hibiscus manihot L.flower[D].Lanzhou:Gansu Agricultural University,2012
[16] 兰蓉,李浡,刘卉,等.金花葵总黄酮的提取和含量测定[J].江苏农业科学,2012,40(12):280 LAN R,LI B,LIU H,et al.Extraction and determination of total flavonoids from Hibiscus manihot L.[J].Jiangsu Agric Sci,2012,40(12):280
[17] 危晴,兰蓉,辛秀兰,等.紫外分光光度法测定金花葵籽中总黄酮的含量[J].安徽农业科学,2012,40(12):7050 WEI Q,LAN R,XIN XL,et al.Determination of total flavonoids content in golden kwai seed by ultraviolet spectrophotometry[J].J Anhui Agric Sci,2012,40(12):7050
[18] 仇燕,庞丽然,李志伟,等.菜芙蓉醇提物金丝桃苷含量测定及对肿瘤细胞生长抑制作用[J].安徽农业科学,2011,39(14):8331 QIU Y,PANG LR,LI ZW,et al.Determination of hyperoside in ethanolic extract of Abelmoschus manihot(L.)Medic and its growth inhibitory effects on tumor cells[J].J Anhui Agric Sci,2011,39(14):8331
[19] 彭志兵,吴正平.高效液相色谱-质谱分析金花葵花中金丝桃苷[J].安徽农业科学,2008,36(23):10028 PENG ZB,WU ZP.Analysis of hyperoside in flower of Hibiscus manihot L.by HPLC-mass spectrometry[J].J Anhui Agric Sci,2008,36(23):10028

欢迎阅读《药物分析杂志》!您是该文第 96位读者!

药物分析杂志 © 2009
地址:北京天坛西里2号 邮政编码:100050; 电子邮件:ywfx@nicpbp.org.cn

本系统由北京博思汇文数字科技有限公司设计开发 技术服务电话:400-921-9838