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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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超高效亲水色谱快速测定吸烟者尿液中的尿酸和肌酐研究

Study on rapid determination of uric acid and creatinine in urine of smokers by hydrophilic UPLC

作者(英文):
分类号:R917
出版年·卷·期(页码):2019,39 (2):286-290
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立超高效亲水色谱法快速测定吸烟者尿液中肌酐和尿酸的含量。方法:尿液样品采用自主设计的萃取瓶处理,然后以Waters ACQUITY UPLC BEH HILIC液相色谱柱(50 mm×2.1 mm,1.7μm)为固定相,70%乙腈(内含0.1%磷酸二氢氨)为流动相进行超高效液相色谱分离,流速0.5 mL·min-1,检测波长235 nm,进样体积5.0μL,柱温30℃,紫外检测器检测。结果:选定实验条件下,尿酸质量浓度在2.5~500μg·mL-1、肌酐质量浓度在5~1 000μg·mL-1内均具有良好的线性关系(r>0.999 5);尿酸和肌酐的检测下限分别为0.25μg·mL-1与0.22μg·mL-1。在3个不同添加水平下,方法平均回收率在95.6%~102.3%,日内精密度和日间精密度均小于3.4%,具有较好的回收率和重复性。结论:本文所建立的方法能够满足快速、准确地测定吸烟者尿液中尿酸和肌酐的要求。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a rapid method for the determination of uric acid and creatinine in the urine of smoker by hydrophilic UPLC. Methods: The urine samples were treated with a self-designed extraction bottle. Then, the uric acid and creatinine were separated on an Waters ACQUITY UPLC BEH HILIC chromatography column (50 mm×2.1 mm,1.7μm) with 70% acetonitrile (containing 0.1% ammonium di-hydrogen phosphate) as mobile phase, the flow rate was 0.5 mL·min-1, the detection wavelength was 235 nm, the injection volume was 5.0μL,and the column temperature was 30℃,and detected with UV detector. Results: Under the selected experimental conditions, the targets were with good linearity (r>0.999 5) in the range of 2.5-500 μg·mL-1 for uric acid and 5.0-1 000μg·mL-1 for creatinine, and the detection limits of uric acid and creatinine reach 0.25 μg·mL-1 and 0.22μg·mL-1,respectively. The average recovery of the method was 95.6% to 102.3% under three different levels of dosage added, and intra-day and inter-day precision were less than 3.4%, which had good recovery and repeatability. Conclusion: The method established in this study can meet the requirements of rapid and accurate determination of uric acid and creatinine in urine of smokers.

-----参考文献:---------------------------------------------------------------------------------------

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