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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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酒苁蓉HPLC特征图谱及5个苯乙醇苷类成分含量测定

Study on characteristic chromatogram and quantitation method of five components for wine-processed Cistanches Herba

作者(英文):
分类号:R917
出版年·卷·期(页码):2019,39 (2):233-239
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:采用高效液相色谱法建立酒苁蓉的特征图谱,并同时测定5个苯乙醇苷类成分的含量。方法:以松果菊苷、肉苁蓉苷A、毛蕊花糖苷、异毛蕊花糖苷和2'-乙酰基毛蕊花糖苷为对照,采用Agilent EclipseXDB-C18(4.6 mm×250 mm,5 μm)色谱柱,以甲醇为流动相A,0.1%甲酸为流动相B,梯度洗脱(0~15min,15% A → 21% A;15~20 min,21% A → 25% A;20~70 min,25% A → 43% A),流速1.0 mL·min-1,检测波长为330 nm,柱温35℃。结果:建立酒苁蓉特征图谱,70 min内酒苁蓉的主要色谱峰能够达到完全分离,同时标定5个苯乙醇苷类成分,对15批饮片中的5个成分进行含量测定。松果菊苷、肉苁蓉苷A、毛蕊花糖苷、异毛蕊花糖和2'-乙酰基毛蕊花糖苷的质量浓度分别在10.05~1 005 μg·mL-1r=0.999 9,n=6)、2.02~202 μg·mL-1r=0.999 9,n=6)、2.04~204 μg·mL-1r=0.999 9,n=6)、2.01~201 μg·mL-1r=0.999 9,n=6)、2.02~202 μg·mL-1r=0.999 9,n=6)范围内线性关系良好,方法的平均回收率(n=6)分别为99.8%(RSD=0.30%)、99.5%(RSD=1.6%)、100.2%(RSD=0.44%)、99.5%(RSD=0.53%)、99.8%(RSD=0.36%);15批酒苁蓉中上述5个成分含量分别为0.20%~7.38%、0.03%~0.20%、0.03%~0.72%、0.03%~0.75%、0.03%~0.07%。结论:该方法操作简单,重复性好,并能同时测定5个苯乙醇苷类成分含量,具备定性定量双重作用,可为酒苁蓉的质量标准研究提供更加全面的依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC characteristic chromatogram and quantitative method for five components in Cistanches Herba. Methods: With echinacoside, cistanoside A, acteoside, iso-acteoside and 2'-acetyacteoside as reference substances,HPLC analysis was performed on an Agilent Eclipse XDB-C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was methanol-0.1% formic acid solution with gradient elution (0-15 min, 15%A → 21%A; 15-20 min, 21%A → 25%A; 20-70 min, 25%A → 43%A). The flow rate was 1 mL·min-1, the detection wavelength was 330 nm and the column temperature was 35℃. Results: HPLC characteristic chromatogram of Cistanches Herba was established and five characteristic peaks were marked. Five characteristic peaks were simultaneously determined by HPLC within 70 min. The linear ranges of echinacoside, cistanoside A, acteoside, iso-acteoside and 2' -acetyacteoside were 10.05-1005 μg·mL-1(r=0.999 9, n=6), 2.02-202 μg·mL-1(r=0.999 9, n=6), 2.04-204 μg·mL-1(r=0.999 9, n=6), 2.01-201 μg·mL-1 (r=0.999 9, n=6) and 2.02-202 μg·mL-1(r=0.999 9, n=6), respectively. The average recovery rate (n=6) were 99.8%(RSD=0.30%), 99.5%(RSD=1.6%), 100.2%(RSD=0.44%), 99.5%(RSD=0.53%) and 99.8%(RSD=0.36%), respectively. The contents of the five components in 15 batches of Cistanches Herba were 0.20%-7.39%, 0.03%-0.20%, 0.03%-0.72%, 0.03%-0.75% and 0.03%-0.07%, respectively. Conclusion: The proposed specific HPLC characteristic chromatogram and quantitation method of five components for Cistanches Herba is simple and repeatable, which offer reference for quality control of the drug.

-----参考文献:---------------------------------------------------------------------------------------

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