关闭
 
读者在线:用户名 密码
首页 期刊简介 投稿须知 期刊目录 专家风采 编委会 特邀顾问 联系我们 移动出版
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5



刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

访问统计
您是第  2 9 1 2 4 9 8 位浏览者
您当前的位置:首页 >> 正文

2015年版《中华人民共和国药典》收载的鹿源药材中硫酸软骨素含量分析

Analysis of chondroitin sulfate content in deer related medicines in Pharmacopoeia of the People's Republic of China 2015

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (10):1711-1718
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:研究2015年版《中华人民共和国药典》(简称《中国药典》)收载的鹿源药材中硫酸软骨素的含量差异。方法:采用稀碱-浓盐法提取,硫酸软骨素ABC酶酶解样品中的硫酸软骨素,应用高效液相色谱法对样品中的总硫酸软骨素及硫酸软骨素A、B、C含量进行测定;色谱条件:Hypersil SAX强阴离子硅胶色谱柱(4.6 mm×250 mm,5 μm),以2 mol·L-1的氯化钠溶液(盐酸调pH至3.5)和水(盐酸调pH至3.5)为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长232 nm,柱温40℃。结果:梅花鹿鹿茸、鹿角(鹿角脱盘)、鹿角胶(鹿角脱盘胶)、鹿角霜(鹿角脱盘霜)中硫酸软骨素含量分别为2.06、0.46(0.42)、0.93(1.10)、0.22(0.17)g·kg-1;马鹿鹿茸、鹿角(鹿角脱盘)、鹿角胶(鹿角脱盘胶)、鹿角霜(鹿角脱盘霜)中硫酸软骨素含量分别为1.83、0.41(0.34)、0.91(0.87)、0.19(0.15)g·kg-1结论:《中国药典》收载的鹿源药材中硫酸软骨素的含量差异很大,鹿茸中的最高,鹿角胶、鹿角、鹿角霜中的依次递减。

-----英文摘要:---------------------------------------------------------------------------------------

Objective:To study the differences of chondroitin sulfate contents in deer related medicines in Pharmacopoeia of the People's Republic of China(Chinese Pharmacopoeia) 2015.Methods:Chondroitin sulfates from samples was extracted by dilute alkali-concentrated salt method.Then, chondroitin sulfate was digested by chondroitinase ABC.The contents of total chondroitin sulfate and chondroitin sulfate A, B and C in the samples were determined by HPLC.The separation was performed on a Hypersil SAX strong anionic silica column (4.6 mm×250 mm, 5 μm) with 2 mol·L-1 sodium chloride solution (pH adjusted to 3.5 with hydrochloric acid) and water (pH adjusted to 3.5 with hydrochloric acid) as the mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was 232 nm and the column temperature was set at 40℃.Results:The contents of chondroitin sulfate in Cervi Cornu Pantotrichum, Cervi Cornu(antlers plate), Cervi Cornu Colla (antlers plate colla), Cervi Cornu and Degelatinatum(antlers plate degelatinatum) of Cervus nippon Temminck were 2.06, 0.46(0.42), 0.93(1.10) and 0.22(0.17) g·kg-1, respectively.The contents of chondroitin sulfate in Cervi Cornu Pantotrichum, Cervi Cornu(antlers plate), Cervi Cornu Colla (antlers plate colla), and Cervi Cornu Degelatinatum(antlers plate degelatinatum) of Cervus elaphus Linnaeus were 1.83, 0.41(0.34), 0.91(0.87) and 0.19(0.15) g·kg-1, respectively.Conclusion:There are significant differences in the contents of chondroitin sulfate in deer related medicines in Chinese Pharmacopoeia, the contents of chondroitin sulfate in Cervi Cornu Pantotrichum were the highest, the contents of chondroitin sulfate in Cervi Cornu Colla, Cervi Cornu and Cervi Cornu Degelatinatum were descending in turn.

-----参考文献:---------------------------------------------------------------------------------------
[1] 中华人民共和国药典2015年版. 一部[S]. 2015:321 ChP 2015. Vol Ⅰ[S]. 2015:321
[2] 李春义.鹿茸完全再生机制研究进展[J].农业生物技术学报,2017,25(1):1 LI CY. Progress in the mechanism underlying full regeneration of mammalian organ deer antlers[J]. J Agric Biotechnol,2017,25(1):1
[3] 曹胜男,包海鹰.鹿角的化学成分及药理活性研究进展[J]. 经济动物学报,2011,15(4):230 CAO SN,BAO HY. Research progress on chemical constituents and pharmaceutical activities of antlers[J]. J Econ Animl,2011,15(4):230
[4] 钱璟,吉静娴,黄凤杰,等.鹿角脱盘活性物质的研究与开发利用[J]. 药物生物技术,2009,16(3):283 QIAN J,JI JX,HUANG FJ,et al. Study and utilization of the active substance in antler plate[J]. Pharm Biotechnol,2009,16(3):283
[5] 鲍悦,高久堂,孙佳明,等.中药鹿角胶的研究进展[J]. 吉林中医药,2016,36(2):173 BAO Y,GAO JT,SUN JM,et al. Research progress of traditional Chinese medicine Colla Corni Cervi[J]. Jilin J Tradit Chin Med,2016,36(2):173
[6] ZHOU QL,GUO YJ,WANG LJ,et al. Velvet antler polypeptides promoted proliferation of chondrocytes and osteoblast precursors and fracture healing[J]. Acta Pharmaol Sin,1999,20(3):279
[7] ⅡDA J,MEIJNE AM,KNUTSON JR,et al. Cell surface chondroitin sulfate proteoglycans in tumor cell adhesion,motility and invasion[J]. Sem Cancer Biol,1996,7(3):155
[8] PUMPHREY CY,THEUS AM,LI S,et al. Neoglycans,carbodiimide-modified glycosam in oglycosam in oglycans:a new class of anticancer agents that inhibits cancer cell proliferation and induces apoptosis[J]. Cancer Res,2002,62(13):3722
[9] 赵虎,周庭银.骨质中硫酸软骨素蛋白多糖的类型和特征[J]. 中国生物化学与分子生物学报,2000,16(2):231 ZHAO H,ZHOU TY. Characteristics of chondroitin sulfated proteoglycans in the matrix phase of bones[J]. Chin J Biochem Mol Biol,2000,16(2):231
[10] CAMPO RD,ROMANO JE. Changes in cartilage proteoglycans associated with calcification[J]. Calcif Tissue Int,1986,39(3):175
[11] BOSKEY AL,STINER D,DOTY SB,et al. Studies of mine realization in tissue culture:optiomal conditions for carilage calcification[J]. Bone Miner,1992,16(1):11
[12] DENHOLM EM,LIN YQ,SILVER PJ,et al. Antitumor activities of chondroitinase AC and chondroitinase B:inhibition of angiogenesis,proliferation and invasion[J]. Eur J Pharmacol,2001,416(3):213
[13] 徐传屯,关瑞章,郑江.硫酸软骨素测定方法的研究进展[J]. 集美大学学报(自然科学版),2008,13(1):54 XU CT,GUAN RZ,ZHENG J. Research progress of the determination methods of chondroitin sulfate[J]. J Jimei Univ(Nat Sci),2008,13(1):54
[14] 任丽萍,于海洲,宋玉娟,等.酶解液相色谱法测定硫酸软骨素钠含量[J]. 药物分析杂志,2012,32(7):1246 REN LP,YU HZ,SONG YJ,et al. Enzymolysis-HPLC determination of the content of chondroitin sulfate sodium[J]. Chin J Pharm Anal,2012,32(7):1246
[15] 李和平,王春生.生态养鹿[M]. 北京:中国农业出版社,2011:89 LI HP,WANG CS. Ecological Deer[M]. Beijing:China Agriculture Press,2011:89
[16] 王玉田.动物性副产品加工利用[M]. 北京:化学工业出版社,2009:152 WANG YT. Processing and Utilization of Animal by-products[M]. Beijing:Chemical Industry Press,2009:152
[17] 熊双丽,李安林,张晓娟. 猪肺管硫酸软骨素的提取分离与抗氧化活性研究[J]. 食品工业科技,2014,35(17):93 XIONG SL,LI AL,ZHANG XJ. Purification and antioxidant activity of chondroitin sulfate from pig lung trachea[J]. Sci Technol Food Ind,2014,35(17):93
[18] 陆钊,刘小虎,刘田,等. 猪硫酸软骨素的制备和含量测定[J]. 第四军医大学学报,2009,30(20):2172 LU Z,LIU XH,LIU T,et al. Synthesis and determination of pig chondroitin sulfate[J]. J Fourth Mil Med Univ,2009,30(20):2172
[19] 中华人民共和国药典2015年版. 二部[S]. 2015:1340 ChP 2015. Vol Ⅱ[S]. 2015:1340
[20] 曹译,王瑞林,张敏,等. 鹿茸硫酸软骨素蛋白聚糖的分离纯化研究[J]. 生物学杂志,2006,23(5):30 CAO Y,WANG RL,ZHANG M,et al. Study of isolation and identification of chondroitin sulfate proteoglycan in pliose antler[J]. J Biol,2006,23(5):30

欢迎阅读《药物分析杂志》!您是该文第 121位读者!

药物分析杂志 © 2009
地址:北京天坛西里2号
邮政编码:100050; 技术支持:010-60213898

电子邮件:ywfx@nicpbp.org.cn