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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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UFLC法同时测定黄芪桂枝五物汤中4个活性成分的含量

Simultaneous determination of four active constituents in Huangqi Guizhi Wuwu decoction by UFLC

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (10):1683-1688
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立超快速液相色谱法同时测定黄芪桂枝五物汤(黄芪、桂枝、芍药、生姜、大枣)中毛蕊异黄酮苷、芍药苷、芍药内酯苷和桂皮酸的含量,为黄芪桂枝五物汤的质量控制提供依据。方法:采用Shim-Pack XR-ODS色谱柱(75 mm×3.0 mm,2.2 μm);流动相为0.1%磷酸水溶液(A)-乙腈(B),梯度洗脱(0~5 min,10% B→20% B;5~10 min,20% B→25% B;10~15 min,25% B→35% B;15~18 min,35% B→ 43% B),平衡时间为5 min;流速为0.4 mL·min-1;柱温为35℃;检测波长分别为232 nm(毛蕊异黄酮苷、芍药苷、芍药内酯苷)和290 nm(桂皮酸);进样量为5 μL。结果:毛蕊异黄酮苷、芍药苷、芍药内酯苷和桂皮酸质量浓度分别在1~50 μg·mL-1r=0.999 7)、10~500 μg·mL-1r=0.999 8)、2.5~125 μg·mL-1r=0.999 8)和5~250 μg·mL-1r=0.999 7)范围内与峰面积呈良好的线性关系;平均回收率分别为96.9%、99.4%、98.5%和98.9%。6批样品中上述4个成分的含量范围分别为0.185~0.221、18.80~23.49、4.00~4.92和0.644~0.681 mg·mL-1结论:本法可用于黄芪桂枝五物汤的质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective:To develop an ultra-fast liquid chromatography (UFLC) method for simultaneous determination of four active constituents(calycosin-7-O-β-D-glucoside, paeoniflorin, albiflorin and cinnamic acid) in Huangqi Guizhi Wuwu decoction. Methods:Chromatographic separation was achieved on a Shim-Pack XR-ODS column (75 mm×3.0 mm, 2.2 μm). The mobile phase consisted of water (containing 0.1% phosphoric acid) (A) and acetonitrile(B) with gradient elution(0-5 min, 10%B→20%B;5-10 min, 20%B→25%B; 10-15 min, 25%B→35%B;15-18 min, 35%B→43%B) at a flow rate of 0.4 mL·min-1 and the equilibration time was 5 min. Column temperature was maintained at 35℃ and the injection volume was 5 μL. Calycosin-7-O-β-D-glucoside, paeoniflorin and albiflorin were determined at 232 nm, and cinnamic acid was determined at 290 nm. Results:The linear ranges were 1.0-50 μg·mL-1(r=0.999 7) for calycosin-7-O-β-D-glucoside, 10-500 μg·mL-1(r=0.999 9) for paeoniflorin, 2.5-125 μg·mL-1(r=0.999 8) for albiflorin and 5.0-250 μg·mL-1(r=0.999 7) for cinnamic acid. The average recoveries (n=9) of calycosin-7-O-β-D-glucoside, paeoniflorin, albiflorin and cinnamic acid were 96.9%, 99.4%, 98.5% and 98.9%, respectively. The content ranges of four components in six batches of samples were 0.185-0.221 mg·mL-1 for calycosin-7-O-β-D-glucoside, 18.80-23.49 mg·mL-1 for paeoniflorin, 4.00-4.92 mg·mL-1 for albiflorin and 0.644-0.681 mg·mL-1 for cinnamic acid. Conclusion:The developed method is suitable for the quality control of Huangqi Guizhi Wuwu decoction.

-----参考文献:---------------------------------------------------------------------------------------

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