关闭
 
读者在线:用户名 密码
首页 期刊简介 投稿须知 期刊目录 专家风采 编委会 特邀顾问 联系我们 移动出版
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5



刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

访问统计
您是第  3 0 6 2 4 8 1 位浏览者
您当前的位置:首页 >> 正文

猴真菌监测分析

Monitoring and analysis of monkey fungi

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (9):1511-1522
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:了解猴真菌携带情况,为猴致病性真菌感染防控提供科学依据。方法:用真菌培养、TaqMan-MGB探针实时荧光定量PCR、普通PCR、基因克隆和测序技术,进行猴的真菌监测分析。对所有真菌分离株进行抗真菌药敏试验。结果:从全国几个不同厂家122只猴中分离获得71株真菌,形态和分子鉴定确证这些真菌为阿萨希毛孢子菌、圆形毛孢子菌、白色念珠菌、黄曲霉、烟曲霉、黑曲霉、焦曲霉、杂色曲霉、聚多曲霉、匿名曲霉、产黄青霉、草酸青霉、变幻青霉、宛氏拟青霉、小刺青霉、绳状青霉、卷枝毛霉、多变根毛霉、伞枝犁头霉、球毛壳霉、热带头梗霉、节菱孢霉菌、暗孢节菱孢菌、链格孢、松色二孢菌。结果表明,猴真菌种类比较丰富,获得真菌11属25种,毛孢子菌属、曲霉属、青霉属、毛霉属、念珠菌属真菌在这些致病真菌中占主导地位。抗真菌药敏试验分析结果显示:这些真菌分离株对制霉菌素、两性霉素B、氟康唑、咪康唑、酮康唑、氟胞嘧啶、特比萘芬已产生了耐药性。结论:联合使用真菌培养、TaqMan-MGB探针实时荧光定量PCR、普通PCR、基因克隆和测序技术能有效分析猴真菌。猴的真菌大多为人兽共患病病原体。研究结果为我国猴真菌监测提供参考数据,为致病性真菌的侵袭防治提供科学依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To understand the carrying condition of monkey fungus, to provide scientific basis for the prevention and control of pathogenic fungal infection in monkeys. Methods: The monkey fungi were monitored and analyzed by means of fungal culture, TaqMan-minor groove binder probe based real-time fluorescence quantitative polymerase chain reaction (TaqMan-MGB probe RTFQ-PCR), conventional PCR, gene cloning and sequencing techniques. Antifungal susceptibility test was carried out for all fungi isolates. Results: Seventy-one fungi were isolated from 122 monkeys from several different manufacturers in China. Morphological and molecular identification confirmed these fungi as Trichosporon asahii, Trichosporon montevideense, Candida albicans, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus ustus, Aspergillus versicolor, Aspergillus sydowii, Aspergillus nomius, Penicillium chrysogenum, Penicillium oxalicum, Penicillium variabile, Paecilomyces variotii, Penicillium spinulosum, Penicillium funiculosum, Mucor circinelloides, Rhizomucor variabilis, Absidia corymbifera, Chaetomium globosum, Cephaliophora tropica, Arthrinium ovatum, Arthrinium phaeospermum, Alternaria alternata and Diplodia pinea. The results showed that the species of monkey fungi were richness and diverse. Twenty-five species of fungi which belong to 11 genera were obtained. The genus Trichosporon, Aspergillus, Penicillium, Mucor and Candida were dominant in these pathogenic fungi. The determination of the antifungal sensitivity test showed that these fungal strains were resistant to nystatin, amphotericin B, fluconazole, miconazole, ketoconazole, fluorocytosine and terbinafine. Conclusion: Combined use of fungal culture, TaqMan-MGB probe RTFQ-PCR, conventional PCR, gene cloning and sequencing technology can effectively analyze the monkey fungi. Most of monkey fungi were zoonotic pathogens. The results provide reference data for monkey fungus monitoring in China and provide scientific basis for the prevention and treatment of pathogenic fungi.

-----参考文献:---------------------------------------------------------------------------------------
[1] GB/T 14926.4-2001实验动物·皮肤病原真菌检测方法[S].2001 GB/T 14926.4-2001 Laboratory Animal·Method for Examination of Pathogenic Dermal Fungi[S].2001
[2] 魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979:13 WEI JC.Handbook of Fungi Identification[M].Shanghai:Shanghai Science and Technology Press,1979:13
[3] 王端礼.医学真菌学:实验室检验指南[M].北京:人民卫生出版社,2005:103 WANG DL.Medical Mycology-Guide to Laboratory Examination[M].Beijing:People's Medical Publishing House,2005:103
[4] 高正琴,岳秉飞.中国新疆灰仓鼠真菌多样性[J].中国人兽共患病学报,2014,30(7):709 GAO ZQ,YUE BF.Diversity of fungi derived from the Cricetulus migratorius from the Xinjiang region of China[J].Chin J Zoonoses,2014,30(7):709
[5] WHIET TJ,BRUNS T,LEE S,et al.Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics.PCR Protocols:A Guide to Methods and Application[M].New York:Academic Press,1990:315
[6] FLIEGEROVÁ K, MRÁZEK J, VOIGT K.Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP[J].Folia Microbiol(Praha), 2006,51(4):273
[7] O'DONNELL K.Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum(Gibberella pulicaris)[J].Curr Genet, 1992,22(3):213
[8] DAGAR SS,KUMAR S,MUDGIL P,et al.D1/D2 domain of large subunit rDNA for differentiation of Orpinomyces spp.[J].Appl Environ Microbiol, 2011,77(18):6722
[9] 高正琴,岳秉飞.白色念珠菌TaqMan-小沟结合物探针实时荧光定量聚合酶链式反应快速检测及抗真菌药物敏感性分析[J].药物分析杂志,2017,37(6):1071 GAO ZQ,YUE BF.Rapid detection of Candida albicans by real-time fluorescent quantitative polymerase chain reaction using TaqMan-minor groove binder probe and analysis of antifungal susceptibility[J].Chin J Pharm Anal,2017,37(6):1071
[10] National Committee for Clincial Laboratory Standards.Method for Antifugal Disk Diffusion Susceptibility Testing of Yeasts:Approved Guideline M44-A[S].2004
[11] ESPINEL-INGROFF A,CANTON E,FOTHERGILL A,et al.Quality control guidelines for amphotericin B,Itraconazole,posaconazole,and voriconazole disk diffusion susceptibility tests with nonsupplemented Mueller-Hinton Agar(CLSI M51-A document) for nondermatophyte filamentous fungi[J].J Clin Microbiol,2011,49(7):2568
[12] TAYLOR LH,LATHAM SM,WOOLHOUSE ME.Risk factors for human disease emergence[J].Philos Trans R Soc Lond B Biol Sci,2001,356(1411):983
[13] WOOLHOUSE ME,GOWTAGE-SEQUERIA S.Host range and emerging and reemerging pathogens[J].Emerg Infect Dis,2005,1(12):1842
[14] 于恩庶,黄丰,潘亮,等.当今人兽共患病病原体分类[J].中国人兽共患病学报,2006,22(6):485 YU ES,HUANG F,PAN L,et al.Zoonotic pathogens species[J].Chin J Zoonoses,2006,22(6):485
[15] de ALMEIDA JÚNIOR JN,HENNEQUIN C.Invasive trichosporon infection:a systematic review on a re-emerging fungal pathogen[J].Front Microbiol,2016(7):1629
[16] SALVATORI O,PURI S,TATI S,et al.Innate immunity and Saliva in Candida albicans-mediated oral diseases[J].J Dent Res, 2016,95(4):365
[17] WIJESURIYA TM, KOTTAHACHCHI J, GUNASEKARA TD,et al.Aspergillus species:an emerging pathogen in onychomycosis among diabetics[J].Indian J Endocrinol Metab,2015,19(6):811.
[18] 佟飞,吴志坚,石寒,等.一起进食含黄曲霉毒素饲料引起食蟹猴急性中毒死亡病例报告[J].广西预防医学,2003,9(5):288 TONG F,WU ZJ,SHI H,et al.A case of acute poisoning death of Macaca fascicularis caused by aflatoxin feed[J].Guangxi Prev Med,2003,9(5):288
[19] MOORE GG,MACK BM,BELTZ SB.Genomic sequence of the aflatoxigenic filamentous fungus Aspergillus nomius[J].BMC Genomics,2015,16(1):551
[20] ABUALI MM,POSADA R,del TORO G,et al.Rhizomucor variabilis var.regularior and Hormographiella aspergillata infections in a leukemic bone marrow transplant recipient with refractory neutropenia[J].J Clin Microbiol,2009,47(12):4176
[21] BELFIORI R,TERENZI A,MARCHESINI L,et al.Absidia corymbifera in an immune competent accident victim with multiple abdominal injuries:a case report[J].BMC Infect Dis,2007,7:46
[22] TWAROCH TE,CURIN M,STERFLINGER K,et al.Specific antibodies for the detection of Alternaria allergens and the identification of cross-reactive antigens in other fungi[J].Int Arch Allergy Immunol,2016,170(4):269

欢迎阅读《药物分析杂志》!您是该文第 217位读者!

药物分析杂志 © 2009
地址:北京天坛西里2号
邮政编码:100050; 技术支持:010-60213898

电子邮件:ywfx@nicpbp.org.cn