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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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猴头菌丝固体培养物中3个核苷类成分含量测定及HPLC指纹图谱研究

Content of 3 nucleosides and HPLC fingerprint of solid culture of Hericium mycelium

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (4):657-664
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:对猴头菌丝固体培养物中尿苷、鸟苷、腺苷3个核苷类成分进行含量测定,同时建立HPLC指纹图谱。方法:采用SunFireTM C18色谱柱(4.6 mm×250 mm,5 μm),流动相为甲醇-水,梯度洗脱,流速1.0 mL·min-1,检测波长260 nm,柱温30℃。采用中药色谱指纹图谱相似度评价系统软件(2004A)进行指纹图谱分析。结果:10批猴头菌丝固体培养物样品中尿苷、鸟苷、腺苷3个核苷类成分线性范围分别为2.4~38.9、1.9~29.6、1.7~26.9 μg·mL-1,平均加样回收率分别为97.8%、98.4%、99.0%,含量分别在127.17~481.88,68.36~282.36,91.10~280.47 μg·g-1范围内。建立了10个批次猴头菌丝固体培养物的共有图谱,提取了21个色谱峰作为指纹图谱共有峰,指纹图谱相似度在0.93以上。结论:建立的猴头菌丝固体培养物含量测定及指纹图谱方法简便可靠,重复性好,可为猴头菌丝固体培养物质量控制和评价提供依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To determine 3 nucleosides in solid culture of Hericium mycelium including uridine,guanosine and adenosine,and to establish the HPLC fingerprint.Methods: The separation was developed on a SunFireTM C18 column(4.6 mm×250 mm,5 μm)by gradient elution with methanol and water at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 260 nm and the column temperature was set at 30℃.The data calculation was performed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A).Results: Good linearities of uridine,guanosine and adenosine were observed in the ranges of 2.4-38.9,1.9-29.6 and 1.7-26.9 μg·mL-1,respectively.The average recoveries were 97.8%,98.4% and 99.0%,respectively.The content ranges of uridine,guanosine and adenosine were 127.17-481.88,68.36-282.36 and 91.10-280.47 μg·g-1,respectively.The common pattern was established based on fingerprints of 10 batches of solid culture of Hericium mycelium and 21 chromatographic peaks were designated as the common peaks.The similarities of samples were above 0.93.Conclusion: The established methods are simple,reliable and repeatable,which can provide the basis for the evaluation and control of the quality of solid culture of Hericium mycelium.

-----参考文献:---------------------------------------------------------------------------------------
[1] HAN ZH,YE JM,WANG GF.Evaluation of in vivo antioxidant activity of Hericium erinaceus polysaccharides[J].Int J Biol Macromol,2013,52:66
[2] 李巧珍,刘朝贵,于海龙,等.猴头菌不同生长发育期粗蛋白、粗多糖含量及水溶性粗多糖体外免疫活性[J].食用菌学报,2011,18(4):73 LI QZ,LIU CG,YU HL,et al.Protein,polysaccharide and β-glucan levels in Hericium erinaceus fruit bodies harvested at different development stages and the effect of extracted polysaccharide on NO production by RAW264.7 cells[J].Acta Edulis Fungi,2011,18(4):73
[3] 周帅,薛俊杰,刘艳芳,等.高效阴离子色谱-脉冲安培检测法分析食用菌中海藻糖、甘露醇和阿糖醇[J].食用菌学报,2011,18(1):49 ZHOU S,XUE JJ,LIU YF,et al.Determination of arabitol,trehalose and mannitol in the fruit bodies of edible fungi using high performance anion chromatography-pulsed amperometric detection(HAPEC-PAD)[J].Acta Edulis Fungi,2011,18(1):49
[4] LI W,ZHOU W,CHA JY,et al.Sterols from Hericium erinaceum and their inhibition of TNF-alpha and NO production in lipopolysaccharide-induced RAW 264.7 cells[J].Phytochemistry,2015,115:231
[5] WANG K,BAO L,QI Q,et al.Erinacerins C-L,isoindolin-1-ones with α-glucosidase inhibitory activity from cultures of the medicinal mushroom Hericium erinaceus[J].J Nat Prod,2015,78(1):146
[6] LU QQ,TIAN JM,WEI J,et al.Bioactive metabolites from the mycelia of the basidiomycete Hericium erinaceum[J].Nat Prod Res,2014,28(16):1288
[7] 汤俊,程敏,鲁静,等.三种药用真菌核苷类成分的分析及含量比较[C]//2008年中国药学会学术年会暨第八届中国药师周论文集.石家庄:中国药学会学术会务部,2008:474 TANG J,CHENG M,LU J,et al.Profiling and determination of nucleosides in several medicinal fungi by HPLC method[C]//The Proceedings of 2008 Academic Annual Conference of Chinese Pharmaceutical Association and the Eighth China Pharmacists Week.Shijiazhuang:Academic Meeting Affairs Department of Chinese Pharmaceutical Association,2008:474
[8] 毕春洋,李国源,陈婷,等.猴头菌丝固体培养物质量标准研究[J].广东药学院学报,2016,32(6):724 BI CY,LI GY,CHEN T,et al.Study on quality standard for Hericium erinaceus with solid culture[J].J Guangdong Pharm Univ,2016,32(6):724
[9] 郭艳艳,李华,刘钟栋.猴头菌属化学成分及药理活性研究进展[J].农产品加工·学刊,2010,4(8):77 GUO YY,LI H,LIU ZD.Phytochemical components and their pharmacological action of Hericium[J].Acad Period Farm Prod Proce,2010,4(8):77
[10] 王晓玉,蒋秋燕,凌沛学,等.猴头菌活性成分及药理作用研究进展[J].中国生化药物杂志,2010,31(1):70 WANG XY,JIANG QY,LING PX,et al.Research progress in the effective components of Hericium erinaceus and its pharmacological actions[J].Chin J Biochem Pharm,2010,31(1):70
[11] LE MOINE O,QUERTINMONT E,GULBIS B,et al.Blunted anti-inflammatory response to adenosine in alcoholic cirrhosis[J].J Hepatol,1999,31(3):457
[12] 岳显可,曹岗,吴瑶,等.HPLC法测定猴头菇核苷类成分腺苷的含量[J].实用药物与临床,2015,18(4):432 YUE XK,CAO G,WU Y,et al.Determination of nucleosides of adenosine in Hericium erinaceus by HPLC[J].Pract Pharm Clin Remed,2015,18(4):432
[13] 李洁莉,陆玲,陈坤,等.猴头菌及其药物制品腺苷等药效成分分析[J].中国食用菌,2002,21(3):32 LI JL,LU L,CHEN K,et al.Studies on adenosine etc.composition with medicinal effects of Hericium erinaceus and its pharmaceutical products[J].Edible Fungi China,2002,21(3):32
[14] 顾慧芬,庄意丽,张梦玲.HPLC法测定猴头菌片中腺苷[J].中成药,2012,34(7):1405 GU HF,ZHUANG YL,ZHANG ML.Determination of adenosine in Houtoujun tablets by HPLC[J].Chin Tradit Pat Med,2012,34(7):1405
[15] 周静安.RP-HPLC测定复方猴头颗粒剂中腺苷的含量[J].中成药,2005,27(2):213 ZHOU JA.Determination of adenosine in compound Houtou granules by HPLC[J].Chin Tradit Pat Med,2005,27(2):213
[16] 戚雁飞.HPLC测定复方猴头胶囊中腺苷的含量[J].中成药,2002,24(3):181 QI YF.Determination of adenosine in compound Houtou capsules by HPLC[J].Chin Tradit Pat Med,2002,24(3):181
[17] 綦天华,丁石博,王再林,等.小刺猴头菌水溶性小分子提取物HPLC指纹图谱探讨[J].中国生化药物杂志,2012,33(1):8 QI TH,DING SB,WANG ZL,et al.Discussion on HPLC fingerprint of water-soluble small molecule extract of Hericium caput-medusae[J].Chin J Biochem Pharm,2012,33(1):8
[18] 陈婷,杨军辉,李俊松,等.猴头菌丝固体培养物及胃乐宁片低聚糖部位的HPLC-ELSD指纹图谱[J].中成药,2017,39(6):1211 CHEN T,YANG JH,LI JS,et al.HPLC-ELSD fingerprints of oligosaccharide sites from mycelia of Hericium erinaceum solid culture and Weilening tablets[J].Chin Tradit Pat Med,2017,39(6):1211
[19] 石博,李雨亭,綦天华,等.小刺猴头菌发酵液小分子指纹图谱与多糖含量相关性研究[J].菌物研究,2012,10(4):244 SHI B,LI YT,QI TH,et al.Study on the correlation of small molecule fingerprints and polysaccharide content of Hericium caput-medusae fermented liquid[J].J Fungal Res,2012,10(4):244

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