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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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基于UPLC-MS的牡丹皮药材质量评价研究

Study on quality evaluation of Cortex Moutan based on UPLC-MS

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (4):609-617
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立超高效液相色谱-三重四极杆质谱串联法(UPLC-TQ-MS法)同时测定牡丹皮中18个化学成分,并比较其差异。方法:采用Acquity UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7 μm),流动相为0.1%甲酸水溶液-乙腈,梯度洗脱,流速0.4 mL·min-1,柱温35℃,采用多反应监测(MRM)方法进行质谱扫描。结果:18个待测成分的浓度与峰面积线性关系良好(0.992 3 < r2 < 0.999 9)。利用正交偏最小二乘判别分析(OPLS-DA)绘制OPLS-DA得分图及VIP图,发现了芍药苷亚硫酸酯、槲皮素、儿茶素、氧化芍药苷、没食子酸、没食子酸甲酯为硫熏与未硫熏样品之间的差异性成分。结论:建立一种全面、准确的牡丹皮药材质量评价方法,同时为鉴别牡丹皮药材是否经硫熏以及质量控制提供参考。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a method for simultaneous determination of 18 chemical constituents in Cortex Moutan by ultra performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-TQ-MS) and to compare the differences between sulfur-smoked and sulfur-free samples.Methods: The determination was performed on a Waters UPLC BEH C18 column (2.1 mm×100 mm,1.7 μm),with gradient elution of water (0.1% acetic acid) and acetonitrile at a flow rate of 0.4 mL·min-1.The column temperature was 35℃.The target compounds were analyzed by multiple reaction monitoring (MRM) mode.Results: The linearities between the concentrations of the tested components and the peak areas were good (0.992 3 < r2 < 0.999 9).The orthogonal partial least squares discriminant analysis (OPLS-DA) score and VIP picture were drawn by OPLS-DA,which indicated that paeoniflorin sulfite,quercetin,catechin,oxypaeoniflora,gallic acid and methyl gallate were the discriminating components between the sulfur smoked and non-sulfur smoked samples of Cortex Moutan.Conclusion: A comprehensive and accurate method for evaluating the quality of Cortex Moutan was established,which could provide reference for the identification of Cortex Moutan with or without sulfur and its quality control.

-----参考文献:---------------------------------------------------------------------------------------
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