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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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薄荷酚类对照提取物HPLC含量测定研究及其在薄荷药材质量控制中的应用

HPLC assay of phenolic reference extract of mint and its application in quality evaluation of the herb

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (4):582-589
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:制备薄荷酚类对照提取物,探讨选择对照提取物替代单体成分对照品对薄荷药材质量控制的可行性。方法:采用色谱分离技术制备薄荷酚类对照提取物,建立以对照提取物为对照的薄荷药材HPLC含量测定方法,使用C18色谱柱(4.6 mm×150 mm,5 μm),流动相为乙腈(A)-0.5%甲酸水溶液(B),梯度洗脱(0~15 min,10% A→21% A;15~20 min,21% A→24% A;20~23 min,24% A→25% A;23~29 min,25% A→26% A;29~40 min,26% A→28% A;40~50 min,28% A→30% A;50~60 min,30% A),流速1 mL·min-1,检测波长296 nm;同时以橙皮苷、香叶木苷、香蜂草苷、蒙花苷及迷迭香酸对照品为对照,对不同批次的酚类对照提取物进行标定,并分别采用对照提取物法和对照品法对薄荷药材进行HPLC含量测定。结果:以对照提取物为对照,橙皮苷、香叶木苷、香蜂草苷、蒙花苷、迷迭香酸的量分别在0.003 45~0.345、0.007 52~0.752、0.001 59~0.159、0.006 74~0.674、0.006 19~0.619 μg范围内线性关系良好;10批对照提取物中上述5个指标性成分的含量分别为5.35%~8.84%、17.09%~25.44%、3.23%~5.41%、12.87%~20.31%、10.72%~20.09%,且不同方法的薄荷药材HPLC含量测得结果基本一致。结论:薄荷酚类对照提取物可用于薄荷药材质量控制,替代单体对照品对药材进行质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To prepare phenolic reference extract (PRE) of mint,and explore its feasibility to replace in reference substances in quality control of traditional Chinese medicine.Methods: PRE of mint was obtained by chromatographic separation as the reference for the assay of the herb.The analysis was performed on a C18 column(250 mm×4.6 mm,5 μm) using acetonitrile(A) and 0.5% acetic acid(B) as the mobile phase with gradient elution(0-15 min,10%A→21%A;15-20 min,21%A→24%A;20-23 min,24%A→25%A;23-29 min,25%A→26%A;29-40 min,26%A→28%A;40-50 min,28%A→30%A;50-60 min,30%A).The flow rate was 1.0 mL·min-1 and the detection wavelength was 296 nm.In the meantime,an HPLC analytical method-based on the PRE was developed to determinate the content of the makers.Results: Based on the PRE,the method had a good linearity in the ranges of 0.003 45-0.345 μg for hesperidin,0.007 52-0.752 μg for diosmin,0.001 59-0.159 μg for didymin,0.006 74-0.674 μg for linarin,0.006 19-0.619 μg for rosmarinic acid,and the quantitative method was validated and the content ranges were 5.35%-8.84% for hesperidin,17.09%-25.44% for diosmin,3.23%-5.41% for didymin,12.87%-20.31% for linarin and 10.72%-20.09% for rosmarinic acid,respectively.The determination results of three methods were compared(reference substances and PRE as reference,respectively),and the results demonstrated that there was no significant difference among the three methods.Conclusion: The presented method which has been proved can be used for the quality control of mint,and will also replace the reference substance to control the quality of traditional Chinese medicine.

-----参考文献:---------------------------------------------------------------------------------------
[1] 陆兔林,翟为民,蔡宝昌,等.对照提取物在中药质量控制中的应用[J].中国中药杂志,2013,38(3):463 LU TL,ZHAI WM,CAI BC,et al.Application of reference extracts in quality control of traditional Chinese medicines[J].China J Chin Mater Med,2013,38(3):463
[2] 王智民,高慧敏,付雪涛,等."一测多评"法中药质量评价模式方法学研究[J].中国中药杂志,2006,31(23):1925 WANG ZM, GAO HM,FU XT,et al.Multi-component quantitation by one marker new method for quality evaluation of Chinese herbal medicine[J].China J Chin Mater Med,2006,31(23):1925
[3] 逄瑜,孙磊,金红宇,等.替代对照品法在中药多指标含量测定中的应用与技术要求探讨[J].药物分析杂志,2013,33(1):169 PANG Y,SUN L,JIN HY,et al.Discussion on application and technical requirements of substitute reference substance method for simultaneous determination of multi-components in traditional Chinese medicine[J].Chin J Pharm Anal,2013,33(1):169
[4] 中华人民共和国药典2010年版.一部[S].2010:285 ChP 2010.Vol Ⅰ[S].2010:285
[5] 中华人民共和国药典2015年版.一部[S].2015:304 ChP 2015.Vol Ⅰ[S].2015:304
[6] 马玲云,马双成.中药标准物质的发展现状与展望[J].中国药事,2010,24(12):1232 MA LY,MA SC.Development and prospect of TCM reference standards[J].Chin Pharm Aff,2010,24(12):1232
[7] 陈沛,金红宇,孙磊,等.对照提取物在中药整体质量控制中的应用[J].药物分析杂志,2016,36(2):185 CHEN P,JIN HY,SUN L,et al.Application of extractive reference substance in holistic quality control of traditional Chinese medicine[J].Chin J Pharm Anal,2016,36(2):185
[8] 周文菊,豆小文,杨美华,等.薄荷及其饮片质量控制研究进展[J].中国中药杂志,2016,41(9):1569 ZHOU WJ,DOU XW,YANG MH,et al.Quality control in Menthae Haplocalycis Herba and its decoction slices[J].China J Chin Mater Med,2016,41(9):1569
[9] 徐晶晶,徐超,刘斌.一测多评法测定薄荷药材中4种黄酮苷的含量[J].中国药学杂志,2014,49(3):234 XU JJ,XU C,LIU B.Simultaneous determination of four flavonoid glycosides in Mentha haplocalyx Briq.by a quantitative analysis of multi-components by single marker[J].,Chin Pharm J,2014,49(3):234
[10] 钟昆芮,张凡,姜艳艳,等.薄荷总黄酮的纯化工艺优选[J].中国实验方剂学杂志,2013,19(6):11 ZHONG KR,ZHANG F,JIANG YY,et al.Optimization of purification technology for total flavonoids from Mentha haplocalyx[J].Chin J Exp Tradit Med Form,2013,19(6):11
[11] 陈向阳,张乐,吴莹,等.LCMS-IT-TOF法快速分析薄荷黄酮部位的主要化学成分[J].北京中医药大学学报,2015,38(8):546 CHEN XY,ZHANG L,WU Y,et al.Rapid analysis of chemical compositions in the flavonoid fraction of Mentha haplocalyx Briq.with LCMS-IT-TOF[J].J Beijing Univ TCM,2015,38(8):546
[12] 徐晶晶,刘斌.基于DPPH、FRAP法的薄荷药材抗氧化谱效关系研究[J].北京中医药大学学报,2015,38(6):405 XU JJ,LIU B.Spectrum-effect relation in antioxidant activity of Menthae Haplocalycis Herba based on DPPH and FRAP assay[J].J Beijing Univ TCM,2015,38(6):405
[13] 李岗,余德顺,杨军,等.超临界CO2萃取薄荷挥发油及其抗氧化能力的研究[J].食品科技,2013,38(1):276 LI G,YU DS,YANG J,et al.The antioxidant capacity of volatile oil from Mentha haplocalyx by supercritical CO2 extraction method[J].Food Sci Technol,2013,38(1):276
[14] 陈智坤,梁呈元,李维林,等.薄荷不同溶剂提取物抗氧化活性的研究[J].食品工业科技,2013,34(3):100 CHEN ZK,LIANG CY,LI WL,et al.Study on the antioxidant activity of different extracts from Mentha canadensis L.[J].Sci Technol Food Ind,2013,34(3):100

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