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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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RP-HPLC测定叶黄素脂质体的含量

RP-HPLC determination of lutein liposomes

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (3):450-454
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立反相高效液相色谱法(RP-HPLC)测定叶黄素脂质体的含量。方法:采用Diamonsil(2) C18(5 μm,250 mm×4.6 mm)色谱柱,以100%纯乙腈为流动相,流速0.6 mL·min-1,检测波长为443 nm,进样量20 μL。结果:叶黄素的线性范围为6.96~69.57 μg·mL-1r=0.999 8),平均加样回收率(n=9)为98.8%。3批样品的含量分别为98.61%、96.17%、100.51%。结论:本方法适用于叶黄素脂质体的含量测定。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an RP-HPLC method for the determination of lutein liposomes.Methods: A Diamonsil(2) C18 column(5 μm,250 mm×4.6 mm) was adopted as the analytical column.The mobile phase consisted of 100% acetonitrile with isocratic elution at a flow rate of 0.6 mL·min-1;the detection wavelength was 443 nm and the injection volume was 20 μL.Results: The linear range was 6.96-69.57 μg·mL-1(r=0.999 8) for lutein and the average recovery(n=9) was 98.8%.The content in three batches of samples was 98.61%,96.17% and 100.51%,respectively.Conclusion: The method can be used for determining the content of lutein liposomes.

-----参考文献:---------------------------------------------------------------------------------------
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