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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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UPLC法结合PLS评价硫磺熏蒸对西洋参皂苷成分的影响

Investigation into the impact of sulfur-fumigation on ginsenosides in Panacis Quinquefolii Radix by UPLC combined with PLS

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (3):432-442
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:评判硫磺熏蒸对西洋参皂苷类成分的影响。方法:采用超高效液相色谱-梯度洗脱法分析测定西洋参甲醇提取液,对获取的硫熏组和无硫组西洋参特征图谱进行偏最小二乘法统计分析,并同时测定人参皂苷Rg1、Re和Rb1的含量,采用UPLC-Q-Tof MS对含量降低的未知皂苷成分进行质谱确证。结果:16批硫熏组与18批无硫组西洋参中人参皂苷Rg1、Re和Rb1的总量均符合中国药典2015年版规定,t检验结果显示其人参皂苷Rg1、Re和Rb1含量及总量无显著性差异;采用偏最小二乘法统计显示硫熏组西洋参与无硫组西洋参样本存在明显分类,硫熏组西洋参人参皂苷Re、Rb1、Rc、Rd含量较无硫组有所降低。含量降低的未知皂苷成分经质谱确证为人参皂苷Rc和Rd。结论:硫磺熏蒸能够引起西洋参中人参皂苷类成分含量的降低。采用UPLC技术结合PLS法能够很好地区分硫磺熏蒸西洋参与未熏蒸西洋参之间的差异,并找到造成差异的皂苷成分,提示该方法可用于硫熏中药材的成分差异分析。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To investigate the impact of sulfur-fumigation on ginsenosides in Panacis Quinquefolii Radix.Methods: A UPLC method coupled with a gradient eluted program was used to analyze the methanol decoction of Panacis Quinquefolii Radix. The partial least squares(PLS)was applied to analyze the chromatograms.The contents of ginsenosides Rg1,Re and Rb1 was also measured.The decreased unknown ginsenosides were interpreted by UPLC-Q-Tof MS/MS.Results: The total contents of ginsenosides Rg1,Re and Rb1 in sulfur-fumigated and non-fumigated Panacis Quinquefolii Radix both met the criterion provided by Chinese Pharmacopeia. t-Test showed there was no significant difference between the contents of ginsenosides Rg1,Re and Rb1 of sulfur-fumigated and that of non-fumigated Panacis Quinquefolii Radix.The total contents of ginsenosides Rg1,Re and Rb1 also had no significant difference between sulfur-fumigated and non-fumigated Panacis Quinquefolii Radix.However,an obvious classification existed between sulfur-fumigated and non-fumigated Panacis Quinquefolii Radix by PLS analysis.The contents of ginsenosides Re,Rb1,Rc and Rd in sulfur-fumigated Panacis Quinquefolii Radix was lower than that in non-fumigated Panacis Quinquefolii Radix.The decreased unknown ginsenosides were confirmed to be ginsenoside Rc and Rd by UPLC-Q-Tof/MS/MS.Conclusion: Sulfur-fumigation would reduce the content of ginsenosides in Panacis Quinquefolii Radix.The UPLC technique combined with PLS could well distinguish the difference between sulfur-fumigated and non-fumigated Panacis Quinquefolii Radix and find the differentiated components.Results suggested that this method can be used for the research on the component changes caused by sulfur-fumigation in Chinese herbs.

-----参考文献:---------------------------------------------------------------------------------------
[1] 中国药典2015年版.一部[S].2015:132 ChP 2015.VolⅠ[S].2015:132
[2] 刘静静,刘晓,李松林,等.硫磺熏蒸中药材及饮片的研究现状[J].中草药,2010,41(8):1043 LIU JJ,LIU X,LI SL,et al.Current situation in studies on traditional Chinese medicinal materials and Yinpian by sulfur-fumigated process[J].Chin Tradit Herb Drugs,2010,41(8):1043
[3] 陆兔林,宁子琬,单鑫,等.硫磺熏蒸对中药材化学成分和药理作用影响的研究进展[J].中国中药杂志,2014,39(15):2796 LU TL,NING ZW,SHAN X,et al.Research situation of effects of sulfur fumigation on quality of traditional Chinese medicine[J].China J Chin Mater Med,2014,39(15):2796
[4] MA XQ,LEUNG AKM CHAN CL et al.UHPLC UHD Q-TOF MS/MS analysis of the impact of sulfur fumigation on the chemical profile of Codonopsis Radix(Dangshen)[J].Analyst,2014,139:505
[5] 蔡皓,乔凤仙,裴科,等.UHPLC-Q-TOF/MS和化学计量学方法分析川芎硫磺熏蒸前后化学成分的变化[J].质谱学报,2017,38(1):127 CAI H,QIAO FX,PEI K,et al.Investigation into differences of chemical constituents between sun-dried and sulfur-fumigated Chuanxiong Rhizoma based on UHPLC-Q-TOF MS and chemometric analysis[J].J Chin Mass Spectrom Soc,2017,38(1):127
[6] 李友连,王珊,朱晶晶,等.硫磺熏蒸对亳菊化学成分的影响[J].中国中药杂志,2015,40(13):2624 LI YL,WANG S,ZHU JJ,et al.Study on effects of sulfur fumigation on chemical constituents of Chrysanthemum morifolium cv.Boju[J].China J Chin Mater Med,2015,40(13):2624
[7] 杨琳,缪宇,殷惠军,等.LC-ESI-MSn法鉴定心悦胶囊中西洋参皂苷类成分[J].中草药,2010,41(12):1942 YANG L,MIAO Y,YIN H,et al.Identification of gingsenosides from Panax quinquefolius in Xinyue capsules by LC-ESI-MSn[J].Chin Tradit Herb Drugs,2010,41(12):1942
[8] 张丹,吴兰芳,王乾,等.不同产地西洋参药材中8 种皂苷类成分含量测定及指纹图谱研究[J].中药材,2016,39(10):2306 ZHANG D,WU LF,WANG Q,et al.Research of the fingerprint and determination of 8 ginsenosides in Panax quinquefolius originated from different regions[J].J Chin Med Mater,2016,39(10):2306
[9] 王露露,孙倩怡,杨慧海,等.模式识别及其在中药质量评价中的应用[J].中草药,2016,23(12):4282 WANG LL,SUN QY,YANG HH,et al.Pattern recognition and its application in quality assessment of Chinese material medica[J].Chin Tradit Herb Drugs,2016,23(12):4282
[10] 刘文慧,李雨,纪玉佳,等.偏最小二乘在中药药性判别中的应用[J].山东大学学报(医学版),2012,50(1):151 LIU WH,LI Y,JI YJ,et al.Partial least squares in the discrimination of traditional Chinese herbal medicine property[J].J Shangdong Univ(Health Sci),2012,50(1):151
[11] 朱尔一.一种适合用于处理中药指纹图谱数据的偏最小二乘法[J].计算机与应用化学,2005,22(8):639 ZHU EY.A kind of PLS method suitable to deal with the fingerprinting data of traditional Chinese medicine[J].Comput Appl Chem,2005,22(8):639
[12] 熊淑雯,章开,陈东,等.指纹图谱技术结合PLS-DA在黄连上清丸质量控制中的应用[J].南昌大学学报(医学版),2015,55(6):15 XIONG SW,ZHANG K,CHEN D,et al.Application of fingerprint and PLS-DA in quality control of Huanglian Shangqing pills[J].J Nanchang Univ(Med Sci),2015,55(6):15
[13] 陈卫,刘林生,顾炳仁,等.基于代谢组学的天舒胶囊质量评价与控制[J].中国药房,2014,25(35):3313 CHEN W,LIU LS,GU BR,et al.Quality evaluation and control of Tianshu capsules based on Omics[J].China Pharm,2014,25(35):3313
[14] 周珊珊,徐金娣,沈红,等.UPLC-Q-TOF-MS/MS结合对照药材法快速筛查硫磺熏蒸商品人参[J].中国中药杂志,2014,39(15):2807 ZHOU SS,XU JD,SHEN H,et al.Development of UPLC-Q-TOF-MS/MS combined with reference herb approach to rapidly screen commercial sulfur-fumigated ginseng[J].China J Chin Mater Med,2014,39(15):2807
[15] JIN X,ZHU LY,SHEN H,et al.Influence of sulphur-fumigation on the quality of white ginseng:a quantitative evaluation of major ginsengsides by high performance liquid chromatography[J].Food Chem,2012,135:1141

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