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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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离子色谱快速检测壳寡糖含量

Rapid detection chirosan oligochitosan by ion chromatography

作者(英文):
分类号:R917
出版年·卷·期(页码):2017,37 (11):1973-1977
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:为了快速准确的进行壳寡糖样品的检测,建立一种分离度好、灵敏度高的壳寡糖离子色谱快速检测方法。方法:实验选用Dionex-CarboPac PA104×50 mm离子交换色谱柱,通过优化柱温、淋洗液配比及浓度等色谱条件,将不同聚合度的壳寡糖在30 min内实现分离。分离后的壳寡糖样品通过计算聚合度为壳2~6糖的含量,检验其样品中壳寡糖的纯度。结果:样品浓度在2.00~10.00 μg·mL-1范围内线性关系良好(r≥0.999 0),精密度实验RSD在0.1%~1.6%之间,样品加标回收率93.2%~105.0%。3种壳寡糖样品中2~6个聚合度壳寡糖的含量之和分别为25.944 μg·g-1、26.406 μg·g-1、25.374 μg·g-1,计算得到纯度为86.5%、88.0%和84.6%,符合国家标准要求。结论:该方法需要样品量少,检验快速,无需衍生,为壳寡糖质量分析、组分分离提供了依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: For the rapid and accurate detection of chitosan oligosaccharide samples, a rapid analysis method with good resolution and high sensitivity was established.Methods: Using Dionex-CarboPac PA104×50 mm ion exchange column, different degrees of polymerization was separated in 30 min by optimizing the column temperature, the ratio and the concentration of eluent.Calculate the content of isolated chitosan oligosaccharide(DP=2-6) to examine the purity of chitosan oligosaccharide.Results: The linear relation of each component was excellent with the range of 2.00-10.00 μg·mL-1(r≥0.999 0), and the relative standard deviations of precision were between 0.1%-1.6%.The recovery rates of the method were between 93.2%-105.0%.The contents of 2-6 degree of polymerization of Chitosan Oligosaccharide in three samples were 25.944 μg·g-1, 26.406 μg·g-1 and 25.374 μg·g-1.So the purities were calculated to be 86.5%、88.0% and 84.6%, and conformed with the national standard.Conclusion: The method detects quickly using few samples and provides the reference for quality analysis and component separation of chitosan oligosaccharide.

-----参考文献:---------------------------------------------------------------------------------------
[1] 汤怀武,王红春,史爱民.农业领域对壳寡糖改性的专利技术发展概述[J].江苏农业科学.2015,43(8):444 TANG HW,WANG HC,SHI AM.Overview of patent technology development of chitosan oligosaccharide modification in agriculture[J].Jiangsu Agr Sci,2015,43(8):444
[2] 杜昱光.壳寡糖的功能研究及应用[M].北京:化学工业出版社,2009:50 DU YG.Research and Application of the Function of Chitosan Oligosaccharide[M].Beijing:Chemical Industry Press, 2009:50
[3] 马镝,吴元华,赵秀香.壳寡糖的制备、分离分析方法及在农业上的应用[J].现代农药,2007,6(2):1 MA D,WU YH,ZHAO XX.Preparation and purification and analysis of chito-oligosaccharide and its application in agriculture[J].Mod Agrochem,2007,6(2):1
[4] 许翔,李吕木,张民扬.壳寡糖的生物学功能及其在畜禽营养中的应用[J].饲料博览,2015,6:31 XU X,LI LM,ZHANG MY.Research progress on biological function of chitosan oligosaccharide and application in livestock nutrition[J].Feed Rev,2015,6:31
[5] 沈献周,沈月新.不同平均分子量的α-壳聚糖的抑菌作用[J].上海水产大学学报,2000,9(2):138 SHEN XZ,SHEN YX.The antibiotic activity of α-chitosan with different molecular weights[J].J Shanghai Fish Univ,2000,9(2):138
[6] 周天,刘晶,周晓梅,等.壳聚糖对作物的生理效应及其增产作用[J].吉林师范大学学报(自然科学版),2003(5):18 ZHOU T,LIU J,ZHOU XM,et al.The effect of chitosan on the physiology and yield increase of crop plants[J].J Jilin Univ(Nat Sci Edit),2003(5):18
[7] 王勤,胡新良,黄雪梅.聚2,2-二甲基三亚甲基碳酸酯与壳寡糖的接枝共聚反应研究[J].广州化工,2014,39(2):27 WANG Q,HU XL,HUANG XM.Synthesis of copolymer of poly(2,2-dimethyl-trimethylene carbonate) grafted onto chitooligosaccharide[J].Guangzhou Chem,2014,39(2):27
[8] 王乃强.我国低聚糖的研发现状与前景[J].精细与专用化学品,2007,15(12):1 WANG NQ.Development situation and prospect of oligosaccharide in China[J].Fine Spec Chem,2007,15(12):1
[9] 李克成,李鹏程,邢荣娥,等.壳寡糖含量的测定方法比较[J].药物分析杂志,2011,31(8)1530 LI KC,LI PC,XING RE,et al.Comparison of methods in determination of chitooligosaccharide[J].Chin J Pharm Anal,2011,31(8):1530
[10] 姜竹茂,功能性低聚糖的发展及应用[J].食品安全导刊,2010,9:58 JIANG ZM.Development and application of functional oligosaccharides[J].China Food Safety,2010,9:58
[11] CENTI S,TATINI F,RATTO F,et al.In vitro assessment of antibody:Conjugated gold nanorods for systemic injections[J].J Nanobiotechnol,2014,12(1):1
[12] SU EP,KIM OH,KWAK JH,et al.Antihyperglycemic effect of short-term arginyl-fructose supplementation in subjects with prediabetes and newly diagnosed type 2 diabetes:randomized, double-blinded,placebo-controlled trial[J].Trials,2014,16(1):1
[13] KROKEIDE IM,SYNSTAD B,GASEIDNES S,et al.Natural substrate assay for chitinases using high-performance liquid chromatography:A comparison with existing assays[J].Anal Biochem,2007,363,128
[14] EL RASSI Z,Recent developments of capillary electrophoresis and capillary electrochromatography of carbohydrate species[J].Electrophoresis,1999,20(15-16):3134
[15] LEE KB,KIM YS,LINHARDT RJ.Capillary zone electrophoresis for the quantitation of oligosaccharides formed through the action of chitinase[J].Electrophoresis,1991,12(9):636
[16] 牟世芬,于泓,蔡亚岐,糖的高效阴离子交换色谱-脉冲安培检测法分析[J].色谱,2009,27(1):667 MOU SF,YU H,CAI YQ.Analysis of sugars by high performance anion-exchange chromatography with pulse damperometric detection[J].Chin J Chromatogr,2009,27(1):667
[17] 蒋慧,李恒,张澜,等.高效液相色谱法测定壳寡糖的含量[J].发酵科技通讯,2016,45(4):232 JIANG H,LI H,ZHANG L,et al.Determination of chitosan oligosaccharide by high performance liquid chromatography[J].Bull Ferment Sci Technol,2016,45(4):232

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