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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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三黄泻心汤质量标志物抑菌生物效价测定

Determination of antibacterial biological titers of Q-Marker in Sanhuang Xiexin decoction

作者: 刘晶晶, 胡晓茹 
作者(英文):
分类号:R917
出版年·卷·期(页码):2019,39 (5):852-858
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:对三黄泻心汤质量标志物抑菌的生物效价进行测定。方法:采用体外抑菌实验,选取与三黄泻心汤及其质量标志物药理作用相关性强的细菌,研究其抑制作用,通过MIC和MBC值的测定及相应抑菌圈直径的测定,计算生物效价。结果:抑菌实验表明,三黄泻心汤水煎剂与质量标志物组对金黄色葡萄球菌、乙型溶血性链球菌抑制作用明显,且质量标志物组抑菌效果明显优于复方水煎液,三黄泻心汤质量标志物组相对于三黄泻心汤水煎剂的生物效价为274.92,即1 g质量标志物组所产生的生物效价相当274.92 g的复方药材。质量标志物组相对于亚胺培南的生物效价为4.387 3,即1 g质量标志物组所产生的生物效价相当于0.438 7 mg所产生的生物效价。结论:基于三黄泻心汤主要用于治疗上呼吸道感染这一疾病,针对三黄泻心汤及质量标志物的抗菌作用开展研究,结果表明三黄泻心汤及质量标志物组有较强的抗菌作用。本工作为新药研发提供一种新思路,为中药质量控制提供了一种新的研究模式。

-----英文摘要:---------------------------------------------------------------------------------------

Objective:To survey and evaluate the biological potency of Q-Maker in Sanhuang Xiexin decoction.Methods:In vitro antibacterial experiment was performed on Sanhuang Xiexin decoction and the Q-Maker using the most relevant bacteria inhibitory effect.The biological titers were calculated through surveying and evaluating the value of MIC, MBC and the diameter of bacteriostatic circle.Results:The bacteriostatic test showed that Sanhuang Xiexin decoction and Q-Maker group had sensitivity to staphylococcus aureus, beta hemolytic streptococcus etc., and the Q-Maker was much better than Sanhuang Xiexin decoction.1 g of Q-Marker group was equivalent to 274.92 g of Sanhuang Xiexin decoction.Additionally, 1 g of Q-Maker group was equivalent to 0.438 7 mg of imipenem.Conclusion:Sanhuang Xiexin decoction is used mainly for the treatment of upper respiratory tract infection, both the Sanhuang Xiexin decoction and the Q-Maker group have strong antibacterial effect.The results of this research provides a new idea for the further research and development of Chinese medicine.This study provides the new research mode for the quality control of traditional Chinese medicine.

-----参考文献:---------------------------------------------------------------------------------------

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