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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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基于LAMP的中药材川贝母真伪鉴别方法的建立

Authentication of Fritillaria cirrhosa based on loop-mediate isothermal amplification

作者(英文):
分类号:R917
出版年·卷·期(页码):2019,39 (3):551-556
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:基于荧光-环状等温扩增技术,建立川贝母快速检测方法,实现中药材川贝母的荧光可视化快速鉴定。方法:依据川贝母物种特异性序列设计5套LAMP引物,并测试方法特异性和灵敏度。结果:引物Set1扩增效率最高;使用该引物建立的川贝母LAMP检测方法仅能够在6种川贝母基源药材中扩增出扩增曲线和荧光变化,在伊贝母、浙贝母、平贝母等7种常见混淆品中无扩增产物,方法特异性良好;方法检测灵敏度为0.1%。结论:本研究建立的基于LAMP的川贝母鉴定方法具有较好的特异性和灵敏度,其快速、操作简单、易观察的优点,填补了中药材川贝母真伪快速筛查的技术空白。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish rapid visual identification method for Fritillaria cirrhosa based on loop-mediated isothermal amplification. Methods: According to the DNA sequence of the target,five sets of primers were designed and screened by amplification efficiency. Specificity and sensitivity of the method was validated. Results: The LAMP detection method established by using primer set1 showed best performance on amplification efficiency, specificity and sensitivity. Turbidity and fluorescence changes could be observed in 6 kinds of Fritillariae, and there was no amplification product in 7 common adulterants. The sensitivity of the LAMP method was 0.1%. Conclusion: The proposed LAMP method is specific, sensitive, accurate and simple and easy to observe, which fills the gap for the authentication of Fritillaria cirrhosa.

-----参考文献:---------------------------------------------------------------------------------------

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