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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法同时测定丹参-当归药对中7个成分的含量

Simultaneous determination of seven components in Radix Salviae Miltiorrhizae-Radix Angelicae Sinensis drug pair by HPLC

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (10):1689-1696
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立HPLC法同时测定丹参-当归药对中丹参素、绿原酸、阿魏酸、迷迭香酸、丹酚酸B、藁本内酯和丹参酮ⅡA 7个成分含量,明确配伍对7个成分含量变化的影响。方法:应用RP-HPLC多波长切换梯度洗脱技术,采用Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5 μm),以乙腈-0.1%磷酸水溶液为流动相,流速1.0 mL·min-1,检测波长280 nm(丹参素、丹参酮ⅡA)、286 nm(丹酚酸B)和320 nm(绿原酸、阿魏酸、迷迭香酸、藁本内酯),柱温25℃。结果:丹参素、绿原酸、阿魏酸、迷迭香酸、丹酚酸B、藁本内酯和丹参酮ⅡA质量浓度分别在7.263~145.3、4.002~200.1、6.433~160.8、10.57~528.3、209.0~1.045×104、111.7~5 584、6.027~301.3 ng·mL-1范围内与峰面积呈良好的线性关系(r=0.999 5~1.000),平均加样回收率在97.1%~101.9%之间(RSD<3%,n=6)。丹参-当归配伍后丹参素和藁本内酯的含量显著增加(P<0.05),而绿原酸、阿魏酸、迷迭香酸、丹酚酸B和丹参酮ⅡA含量无明显变化。3个批次的丹参、当归饮片中上述7个成分含量测定结果分别为0.237 7~0.264 1、0.384 8~0.455 5、0.687 3~0.955 7、1.018~1.768、39.32~54.64、10.65~14.98、1.675~2.608 mg·g-1结论:该方法可用于丹参-当归药对及其制剂的质量控制,并为丹参、当归的临床配伍应用及其研究提供科学依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective:To establish an HPLC method for simultaneous determination of danshensu, chlorogenic acid, ferulic acid, rosmarinic acid, salvianolic acid B, ligustilide and tanshinoneⅡA in Radix Salviae Miltiorrhizae-Radix Angelicae Sinensis drug pair, so as to analyse the variation of the 7 ingredients after compatibility. Methods:The samples were separated on an Eclipse XDB-C18 column(4.6 mm×250 mm, 5 μm) by gradient elution with acetonitrile-0.1% phosphoric acid aqueous solution at a flow rate of 1.0 mL·min-1. The detection wavelength was 280 nm (danshensu and tanshinoneⅡA), 286 nm (salvianolic acid B) and 320 nm (chlorogenic acid, ferulic acid, rosmarinic acid and ligustilide). The column temperature was controlled at 25. Results:Danshensu, chlorogenic acid, ferulic acid, rosmarinic acid, salvianolic acid B, ligustilide and tanshinoneⅡA exhibited good linearity (r=0.999 5-1.000) among the ranges of 7.263-145.3, 4.002-200.1, 6.433-160.8, 10.57-528.3, 209.0-1.045×104, 111.7-5 584 and 6.027-301.3 ng·mL-1, respectively. The average recoveries (RSD<3%, n=6) were between 97.1%-101.9%. After the compatibility, the content of danshensu and ligustilide increased significantly (P<0.05), while there was no significant change in the content of chlorogenic acid, ferulic acid, rosmarinic acid, salvianolic acid B and tanshinoneⅡA. The contents of the above-mentioned compounds in 3 batches of samples were 0.237 7-0.264 1, 0.384 8-0.455 5, 0.687 3-0.955 7, 1.018-1.768, 39.32-54.64, 10.65-14.98 and 1.675-2.608 mg·g-1, respectively. Conclusion:The method can be used for the quality control of Salviae Miltiorrhizae Radix and Angelicae Sinensis Radix drug pair and its preparations, and can provide scientific basis for the clinical compatibility and application of Salviae Miltiorrhizae Radix and Angelicae Sinensis Radix.

-----参考文献:---------------------------------------------------------------------------------------
[1] 刘春慧.基于七情配伍理论的丹参药对临床用药规律研究[J].中医杂志,2016,57(2):118 LIU CH.Research of clinical medication regularity of Radix Salviae Miltiorrhizae based on the theory of compatibility of seven emotions[J].J Tradit Chin Med,2016,57(2):118
[2] 李巧玉,刘杨,包华音,等.近5年丹参化学成分及药理作用研究进展[J].食品与药品,2014,16(2):145 LI QY,LIU Y,BAO HY,et al.Advances in studies on chemical constituents and pharmacological effects of Salvia miltiorrhiza in recent 5 years[J].Food Drug,2014,16(2):145
[3] 梁文仪,陈文静,杨光辉,等.丹参酚酸类成分研究进展[J].中国中药杂志,2016,41(5):806 LIANG WY,CHEN WJ,YANG GH,et al.Research progress in salvianolic acids of Salvia miltiorrhiza[J].China J Chin Mater Med,2016,41(5):806
[4] 李曦,张丽宏,王晓晓,等.当归化学成分及药理作用研究进展[J].中药材,2013,36(6):1023 LI X,ZHANG LH,WANG XX,et al.Research progress in chemical constituents and pharmacological effects of Angelica sinensis[J].J Chin Med Mater,2013,36(6):1023
[5] 张来宾,吕洁丽,陈红丽,等.当归中苯酞类成分及其药理作用研究进展[J].中国中药杂志,2016,41(2):167 ZHANG LB,LÜ JL,CHEN HL,et al.Research progress of structures and pharmacological activities of phthalides from Angelica sinensis[J].China J Chin Mater Med,2016,41(2):167
[6] 程沛,韩东岐,胡伟慧,等.高效液相色谱法同时测定丹参中10种水溶性和4种脂溶性成分的含量[J].药物分析杂志,2015,35(6):991 CHENG P,HAN DQ,HU WH,et al.Simultaneous determination of ten hydrophilic compounds and four lipophilic compounds in Radix Salviae Miltiorrhizae by HPLC[J].Chin J Pharm Anal,2015,35(6):991
[7] 唐登峰,祝明,陈勇,等.RP-HPLC同时测定冠心宁注射液中丹参素、原儿茶醛、阿魏酸、迷迭香酸和丹酚酸B的含量[J].药物分析杂志,2012,32(1):144 TANG DF,ZHU M,CHEN Y,et al.RP-HPLC simultaneous determination of danshensu,protocatechuicaldehyde,ferulic acid,rosmarinic acid,and salvianolicacid B in Guanxinning injection[J].Chin J Pharm Anal,2012,32(1):144
[8] 郜舒蕊,赵志刚,侯俊玲,等,均匀设计法优化丹参中4种活性成分的超声提取工艺[J].中国实验方剂学杂志,2014,20(10):8 GAO SR,ZHAO ZG,HOU JL,et al.Optimization of rapid ultrasonic extraction process of four active components from Salviae Miltiorrhizae Radix et Rhizoma by uniform design[J].Chin J Exp Tradit Med Form,2014,20(10):8
[9] 温学逊,牛研,王书芳,等.当归不同药用部位活性成分含量的HPLC分析方法及特征图谱研究[J].药物分析杂志,2014,34(2):317 WEN XX,NIU Y,WANG SF,et al.HPLC analysis and characteristic chromatograms of active components in different medicinal parts of Angelicae Sinensis Radix[J].Chin J Pharm Anal,2014,34(2):317
[10] 姜鸿,王光函,张颖,等.HPLC法测定火绒草中原儿茶酸、原儿茶醛、绿原酸和咖啡酸[J].中成药,2011,33(11):2023 JIANG H,WANG GH,ZHANG Y,et al.Determination of protocatechuic acid,protocatechuic aldehyde,caffeic acid and chlorogenic acid of edelweiss by HPLC[J].Chin Tradit Pat Med,2011,33(11):2023
[11] 顾志荣,王亚丽,孙宇靖,等.当归中5种成分同时测定与产地质量评价[J].中成药,2014,36(10):2135 GU ZR,WANG YL,SUN YJ,et al.Simultaneous determination and quality evaluation of 5 components in Angelica sinensis[J].Chin Tradit Pat Med,2014,36(10):2135
[12] 张晓,张国庆,顾伯林,等.丹参及其有效成分对骨代谢影响的实验研究进展[J].中国骨质疏松杂志,2015,21(1):112 ZHANG X,ZHANG GQ,GU BL,et al.Experimental research progress of the effect of Salvia miltiorrhiza and its active ingredients on bone metabolism[J].Chin J Osteoporos,2015,21(1):112
[13] BAI B,LI Y.Danshen prevents articular cartilage degeneration via antioxidation in rabbits with osteoarthritis[J].Osteoarthritis Cartilage,2016,24(3):514
[14] 高建林,陈珺,李兵.阿魏酸对体外培养的成骨细胞增殖、分化和矿化功能的影响[J].中国药理学通报,2016,32(10):1479 GAO JL,CHEN J,LI B.Effects of ferulic acid on proliferation,differentiation and mineralization of osteoblasts cultured in vitro[J].Chin Pharmacol Bull,2016,32(10):1479
[15] ZHU MD,ZHAO LX,WANG XT,et al.Ligustilide inhibits microglia-mediated proinflammatory cytokines production and inflammatory pain[J].Brain Res Bull,2014,109:54

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