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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法同时测定连钱草中4个酚酸类成分的含量

HPLC simultaneous determination of four phenolic acids in Herba Glechomae

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (4):643-647
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立同时测定连钱草中咖啡酸、咖啡酰羟基乙酸、迷迭香酸和丹酚酸A 4个酚酸类成分含量的高效液相色谱法。方法:采用CAPCELL PAK C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈(A)-0.1%甲酸水溶液(B)为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长330 nm,柱温30℃。结果:咖啡酸、咖啡酰羟基乙酸、迷迭香酸和丹酚酸A进样量分别在0.034~0.85 μg(r=0.999 8)、0.014~0.35 μg(r=0.999 8)、0.524 5~13.112 5 μg(r=0.999 8)和0.008 4~0.21 μg(r=0.999 9)范围内与峰面积呈现良好的线性关系;平均回收率(n=3)分别为98.2%~99.6%(RSD<0.8)、97.6%~99.4%(RSD<1.3%)、100.2%~100.5%(RSD<1.0%)和98.1%~99.2%(RSD<1.1%);8批样品中咖啡酸、咖啡酰羟基乙酸、迷迭香酸和丹酚酸A含量范围分别为0.238~1.121、0.036~0.180、1.461~11.011和0.042~0.124 mg·g-1结论:该方法可用于连钱草的质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for the simultaneous determination of four phenolic acids in Herba Glechomae including caffeic acid,caffeoylglycolic acid,rosmarinic acid and salvianolic acid A.Methods: The CAPCELL PAK C18 column(250 mm×4.6 mm,5 μm)was used for separation.And the mobile phase consisted of acetonitrile(A)-0.1% formic acid(B)with gradient elution at a flow rate of 1.0 mL·min-1.The detection wavelength was 330 nm and the column temperature was at 30℃.Results: The linear range of caffeic acid,caffeoylglycolic acid,rosmarinic acid and salvianolic acid A were 0.034-0.85 μg (r=0.999 8),0.014-0.35 μg(r=0.999 8),0.524 5-13.112 5 μg(r=0.999 8)and 0.008 4-0.21 μg(r=0.999 9),respectively.The average recoveries(n=3)were 98.2%-99.6%(RSD<0.8%),97.6%-99.4%(RSD<1.3%),100.2%-100.5%(RSD<1.0%)and 98.1%-99.2%(RSD<1.1%),respectively.The contents of caffeic acid,caffeoylglycolic acid,rosmarinic acid and salvianolic acid A in eight batches of samples were 0.238-1.121,0.036-0.180,1.461-11.011 and 0.042-0.124 mg·g-1,respectively.Conclusion: The method can be used to control the quality of the Herba Glechomae.

-----参考文献:---------------------------------------------------------------------------------------
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